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Zonula occludens-1 (ZO-1, batch number AF5145), Beta-Actin antibody (batch number AF7018), Goat Anti-Mouse IgG (H + L) HRP (batch number S0002), and Goat Anti-Rabbit IgG (H + L) HRP (batch number S0001) were obtained from Affinity

In melanoma, the response to ICIs can be enhanced by using Smac mimetics which have a direct impact on immune cells such as B cells, MDSCs, DCs, and cytotoxic T cells (Michie et al., 2020)